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Functional PEG–peptide hydrogels to modulate local inflammation inducedby the pro-inflammatory cytokine TNFα

Paper ID Volume ID Publish Year Pages File Format Full-Text
9024 615 2009 8 PDF Available
Title
Functional PEG–peptide hydrogels to modulate local inflammation inducedby the pro-inflammatory cytokine TNFα
Abstract

Hydrogels are an important class of biomaterials for cell encapsulation and delivery, providing a physical barrier or “immuno-isolation” between the host tissue and encapsulated cells. The semi-permeable gel protects the encapsulated cells from host immune cells and/or antibody recognition while allowing facile diffusion of nutrients. However, a previously un-addressed problem is that highly permissive hydrogels cannot exclude the infiltration of soluble immune-mediators, such as pro-inflammatory cytokines that are highly expressed in wounded environments in vivo. When encountered with pro-inflammatory cytokines, encapsulated cells fail to perform their desired functions. Here, we report the synthesis, characterization, and application of peptide-functionalized, cytokine-antagonizing poly(ethylene glycol) (PEG) hydrogels capable of sequestering the pro-inflammatory cytokine, tumor necrosis factor α (TNFα). Results demonstrate that the survival, function, and differentiation of encapsulated cells (e.g., rat adrenal pheochromocytoma cells – PC12s, mouse pancreatic islets, and human mesenchymal stem cells or hMSCs) are significantly hindered in un-modified PEG hydrogels under in vitro TNFα treatments. In contrast, cells encapsulated in TNFα-antagonizing hydrogels are un-affected by the infiltrated TNFα. This study demonstrates the importance of controlling the availability of pro-inflammatory cytokines in highly permissive hydrogels.

Keywords
Hydrogel; Inflammation; Cytokine; Apoptosis; Mesenchymal stem cells
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Functional PEG–peptide hydrogels to modulate local inflammation inducedby the pro-inflammatory cytokine TNFα
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 28, October 2009, Pages 4907–4914
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us