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In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
9038 615 2009 8 PDF Available
Title
In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration
Abstract

This study investigated the effects of a calcium magnesium silicate bioceramic (akermanite) for bone regeneration in vitro and in vivo, with β-tricalcium phosphate (β-TCP) as a control. In vitro, the human bone marrow-derived mesenchymal stromal cells (hBMSCs) were cultured in an osteogenic medium supplemented with a certain concentration of two bioceramics' extracts for 20 days. An MTT assay showed that akermanite extract promoted proliferation of hBMSC significantly more than did β-TCP extract. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes such as ALP, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) demonstrated that the osteogenic differentiation of hBMSC was enhanced more by akermanite extract than by β-TCP extract. In vivo, a histomorphology analysis and histomorphometry of the two porous bioceramics implants in rabbit femur defect models indicated that both in early- and late-stage implantations, akermanite promoted more osteogenesis and biodegradation than did β-TCP; and in late-stage implantations, the rate of new bone formation was faster in akermanite than in β-TCP. These results suggest that akermanite might be a potential and attractive bioceramic for tissue engineering.

Keywords
Akermanite; β-Tricalcium phosphate; Extract; Mesenchymal stem cell; Rabbit femur defect models
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In vitro and in vivo evaluation of akermanite bioceramics for bone regeneration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 28, October 2009, Pages 5041–5048
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us