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Systemic trafficking of macrophages induced by bone cement particles in nude mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
9066 616 2008 6 PDF Available
Title
Systemic trafficking of macrophages induced by bone cement particles in nude mice
Abstract

Macrophages play an important role in the biological response to wear particles, which can result in periprosthetic osteolysis and implant loosening. In this study, we demonstrate that polymer particles induce systemic trafficking of macrophages by non-invasive in vivo imaging and immunohistochemistry. The distal femora of nude mice were injected with 10% (w/v) Simplex bone cement (BC) suspensions or saline (PBS). Reporter RAW264.7 macrophages which stably expressed the bioluminescent reporter gene fluc, and the fluorescence reporter gene gfp, were injected intravenously. Bioluminescence imaging was performed immediately and periodically at 2-day intervals until day 14. Compared to the non-operated contralateral femora, the bioluminescent signal of femora injected with BC suspension increased 4.7 ± 1.6 and 7.8 ± 2.9-fold at day 6 and 8, respectively. The same values for PBS group were 1.2 ± 0.2 and 1.4 ± 0.5, respectively. The increase of bioluminescence of the BC group was significantly greater than the PBS group at day 8 (p < 0.05) and day 6 (p < 0.1). Histological study confirmed the presence of reporter macrophages within the medullary canal of mice that received cement particles. Modulation of the signaling mechanisms that regulate systemic macrophage trafficking may provide a new strategy for mitigating the chronic inflammatory response and osteolysis associated with wear debris.

Keywords
Macrophage; Polymethylmethacrylate; Wear debris; Non-invasive imaging; Animal model; In vivo test
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 36, December 2008, Pages 4760–4765
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us