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Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex

Paper ID Volume ID Publish Year Pages File Format Full-Text
9075 616 2008 7 PDF Available
Title
Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex
Abstract

Smad3 mediates the intracellular signaling of TGF-β1 superfamily and plays a critical role in the cellular proliferation, differentiation and elaboration of matrix pivotal to cutaneous wound healing. Smad3 antisense oligonucleotides (ASOs) impregnated polyelectrolyte complex (PEC) containing chitosan and sodium alginate was prepared for accelerated wound healing. Physicochemical properties of PEC were characterized by zeta potential, scanning electron microscopy and bioadhesive test. Full-thickness, excisional wounds were made on the dorsum of C57BL6 mice. Then, smad3 ASOs-PEC, PEC alone, smad3 ASOs and gauze dressing were applied to determine concentration of TGF-β1 and collagen in tissues and observe the wound contraction and histology of tissues. Zeta potentials and bioadhesive strengths of ASOs-PEC were increased as the chitosan ratio in PEC. In smad3 ASOs-PEC, the healing process suggested by wound closure and histological observation was faster than other groups because collagen contents increased and level of TGF-β1 decreased. These results demonstrate that the smad3 ASOs-PEC composed of chitosan and sodium alginate could be applied for accelerated wound healing.

Keywords
Smad3; Antisense oligonucleotides (ASOs); Chitosan/alginate polyelectrolyte complex (PEC); Wound healing
First Page Preview
Accelerated wound healing by smad3 antisense oligonucleotides-impregnated chitosan/alginate polyelectrolyte complex
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 36, December 2008, Pages 4831–4837
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering