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Neuronal adhesion and differentiation driven by nanoscale surface free-energy gradients

Paper ID Volume ID Publish Year Pages File Format Full-Text
9082 617 2010 10 PDF Available
Title
Neuronal adhesion and differentiation driven by nanoscale surface free-energy gradients
Abstract

Recent results indicate that, in addition to chemical, spatial and mechanical cues, substrate physical cues such as gradients in surface energy may also impact cell functions, such as neuronal differentiation of PC12 cells. However, it remains to be determined what surface effect is the most critical in triggering PC12 cell differentiation. Here we show that, beyond continuously probing the surface energy landscape of their environment, PC12 cells are highly sensitive to nanoscale chemical heterogeneities. Self-assembled monolayers of alkylsiloxanes on glass were used as a culture substrate. By changing the structure, ordering and chemical nature of the monolayer, the surface energy distribution is altered. While both well-ordered CH3 terminated substrates and bare glass (OH terminated) substrates did not favor PC12 cell adhesion, PC12 cells seeded on highly disordered CH3/OH substrates underwent enhanced adhesion and prompt neuritogenesis by 48 h of culture, without nerve growth factor treatment. These data illustrate that surface free-energy gradients, generated by nanoscale chemical heterogeneities, are critical to biological processes such as nerve regeneration on biomaterials.

Keywords
PC12 cells; Neuronal differentiation; Cell adhesion; Self-assembled monolayers (SAMs); Sum-frequency generation (SFG); Surface energy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 14, May 2010, Pages 3762–3771
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us