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Mediating high levels of gene transfer without cytotoxicity via hydrolytic cationic ester polymers

Paper ID Volume ID Publish Year Pages File Format Full-Text
9127 617 2010 8 PDF Available
Title
Mediating high levels of gene transfer without cytotoxicity via hydrolytic cationic ester polymers
Abstract

Cationic polymers are widely studied as gene-delivery vehicles, but are limited by low transfection due to inhibited release of DNA, and high cytotoxicity from the requisite positive charges. Here, we introduce a hydrolytic cationic ester polymer containing both tertiary and quaternary amines, which packages DNA into nanoparticles and then releases DNA upon hydrolysis. Cells were transfected with these nanoparticles. Luciferase expression from a polymer with the tertiary/quaternary ratio of 1:1 was equal to that obtained using branched polyethylenimine (PEI), and expression from an acidified polymer with the ratio of 3:1 was 20 times higher than branched PEI. These ratios best balance proton sponging from tertiary amines and packaging ability from cations. Importantly, no hydrolysed polymer exhibited cytotoxicity; the zwitterionic nature of the hydrolysed polymer ensured that the quaternary amines in this work do not cause cell death. Hydrolysis is critical for effective and safe gene therapy.

Keywords
Gene transfer; Cell viability; Hydrolysis; Biocompatibility
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Mediating high levels of gene transfer without cytotoxicity via hydrolytic cationic ester polymers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 14, May 2010, Pages 4186–4193
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us