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The control of stem cell morphology and differentiation by hydrogel surface wrinkles

Paper ID Volume ID Publish Year Pages File Format Full-Text
9152 618 2010 8 PDF Available
Title
The control of stem cell morphology and differentiation by hydrogel surface wrinkles
Abstract

In this study, we investigated human mesenchymal stem cell (hMSC) interactions with uniform hydrogels and hydrogels with lamellar or hexagonal surface wrinkles to elucidate our ability to control hMSC morphology and differentiation. Wrinkled hydrogels were prepared from photocurable poly(2-hydroxyethyl methacrylate) (PHEMA) precursor solutions containing ethylene glycol dimethacrylate as a crosslinker, using depth-wise gradients in crosslinking and subsequent buckling with swelling to generate wrinkles. A replica molding process was used to fabricate a series of gels with uniform mechanics, but altered surface wrinkle size and shape. We found that hMSCs attached to lamellar wrinkles spread by taking the shape of the pattern, exhibit high aspect ratios, and differentiate into an osteogenic lineage. In contrast, cells that attached inside the hexagonal patterns remain rounded with low spreading and differentiate into an adipogenic lineage. This work aids in the development of material-based cell culture and scaffold systems to direct stem cell differentiation.

Keywords
Hydrogel; Surface wrinkles; Stem cells; Morphology; Differentiation
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The control of stem cell morphology and differentiation by hydrogel surface wrinkles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 25, September 2010, Pages 6511–6518
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us