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Imaging and cell targeting characteristics of magnetic nanoparticles modified by a functionalizable zwitterionic polymer with adhesive 3,4-dihydroxyphenyl-l-alanine linkages

Paper ID Volume ID Publish Year Pages File Format Full-Text
9160 618 2010 7 PDF Available
Title
Imaging and cell targeting characteristics of magnetic nanoparticles modified by a functionalizable zwitterionic polymer with adhesive 3,4-dihydroxyphenyl-l-alanine linkages
Abstract

Multifunctional magnetic nanoparticles (MNPs) modified by a zwitterionic polymer (pCBMA–DOPA2) containing one poly(carboxybetaine methacrylate) (pCBMA) chain and two 3,4-dihydroxyphenyl-l-alanine (DOPA) residue groups were developed. Results showed that MNPs modified by pCBMA were not only stable in complex media, but also provided abundant functional groups for ligand immobilization. The pCBMA–DOPA2 MNPs had a hydrodynamic particle size of about 130 nm, a strong saturation magnetization of 110.2 emu/g Fe and a high transverse relaxivity of 428 mm−1 s−1. Long-term stability in phosphate-buffered saline (PBS) and 10% NaCl solution was achieved for over six months. Compared to MNPs coated with dextran, pCBMA–DOPA2 MNPs presented better stability in 100% human blood serum at 37 °C. Macrophage cell uptake studies revealed that the uptake ratio of pCBMA–DOPA2 MNPs was much lower than that of dextran MNPs. Furthermore, quantitative analysis results showed that after pCBMA–DOPA2 MNPs were conjugated with a targeting RGD peptide, uptake by human umbilical vein endothelial cell (HUVEC) was notably increased, which was further visualized by magnetic resonance imaging (MRI).

Keywords
Zwitterionic; Iron oxide nanoparticles; Biomimetic linkage; Ultra-low fouling; MRI (magnetic resonance imaging); Targeting
First Page Preview
Imaging and cell targeting characteristics of magnetic nanoparticles modified by a functionalizable zwitterionic polymer with adhesive 3,4-dihydroxyphenyl-l-alanine linkages
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 25, September 2010, Pages 6582–6588
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering