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Mechanical properties of DNA biofilms adsorbed on microcantilevers in label-free biodetections

Paper ID Volume ID Publish Year Pages File Format Full-Text
9168 618 2010 8 PDF Available
Title
Mechanical properties of DNA biofilms adsorbed on microcantilevers in label-free biodetections
Abstract

Biomolecule adsorption is a fundamental process in the design of biosensors. Mechanical/electrical/thermal properties of biofilms have great influences on biodetection signals. The double-stranded DNA (dsDNA) biofilm adhered on microcantilever is treated as a bending beam with a macroscopic elastic modulus in the viewpoint of continuum mechanics. Accounting for hydration force, electrostatic repulsion and conformational entropy, moment-angle diagrams of dsDNA biofilm in pure bending state are depicted with the help of the energy conservation law and a mesoscopic liquid crystal theory presented by Strey et al. An analytical model is provided to predict macroscopic elastic modulus of dsDNA biofilm as a function of nanoscopic properties of dsDNA, packing density, buffer salt concentration and etc. The parameters for microcantilever-DNA system are obtained by curve fitting with Stachowiak’s experimental data based on a modified Stoney’s formula. Elastic modulus grows exponentially with the enhancement of packaging density, but diminishes with the increase of buffer salt concentration, and its order is about 1 ∼ 10 MPa. Conformational entropy is one of predominant factors considered in near-surface system whether in high or low salt consternation.

Keywords
DNA; Adsorption; Biosensor; Biofilm; Mechanical properties; Surface energy
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Mechanical properties of DNA biofilms adsorbed on microcantilevers in label-free biodetections
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 25, September 2010, Pages 6659–6666
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us