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Bioactive enzyme–metal composites: The entrapment of acid phosphatase within gold and silver

Paper ID Volume ID Publish Year Pages File Format Full-Text
9171 619 2009 5 PDF Available
Title
Bioactive enzyme–metal composites: The entrapment of acid phosphatase within gold and silver
Abstract

This paper is concerned with the entrapment of an enzyme within an aggregated metallic matrix and the development of a bioactive enzyme–metal composite. Whereas the use of organic polymers and metal oxides for the preparation of enzymatically active materials is well developed, the third principle enzyme–material combination, namely protein–metal bulk, has not yet been reported. A new methodology for the entrapment of organic molecules and polymers within metals has been employed for the preparation of bioactive acid phosphatase@gold and acid phosphatase@silver, according to which room temperature reduction of the metal cation is carried out in the presence of the enzyme to be entrapped. Protectability of the entrapped enzyme against harsh conditions is shown: the acidic enzyme is kept alive under basic conditions.

Keywords
Bioactivity; Composite; Enzyme; Acid phosphatase; Gold; Silver
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Bioactive enzyme–metal composites: The entrapment of acid phosphatase within gold and silver
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 7, March 2009, Pages 1263–1267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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