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Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
9189 619 2009 7 PDF Available
Title
Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation
Abstract

A new culture substrate was developed for cells to be equibiaxially stretched using fibronectin (Fn)-immobilized temperature-responsive hydrogel. The cells cultured on the gel substrate were equibiaxially stretched with swelling of the gel, which was accompanied by slight changes of temperature. During gel swelling, changes of cell shape were clearly observed by optical microscopy because of high transparency of the gel. ERK was highly and transiently activated by mechanical stimulation whereas focal adhesion kinase (FAK) was not, indicating that mechanical signals were transduced into biochemical signals in cells. We found that cells formed filopodia-like structures in response to mechanical cues, suggesting that mechanical forces facilitated actin polymerization at the peripheral region. In the cytoplasm, paxillin-containing fibrous structures were formed along actin fibers. These results indicate that we can perform both analysis of intracellular signal transduction and observation of cell shapes at high magnification in our method.

Keywords
Hydrogel; Poly(N-isopropylacrylamide); Cell morphology; Mechanical stimuli; Actin; Paxillin
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Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 7, March 2009, Pages 1421–1427
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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