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Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films

Paper ID Volume ID Publish Year Pages File Format Full-Text
9195 620 2009 10 PDF Available
Title
Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films
Abstract

A macrophage culture model was used to investigate the erosion of gamma irradiated poly(trimethylene carbonate) (PTMC) films. When the PTMC films were incubated in the culture medium, but physically separated from the cells by a membrane, no erosion occurred. In contrast, when the J774A macrophages were directly cultured on PTMC films, they adhered to the films and were found to have eroded the polymer surface. Macrophages adhered to gamma irradiated poly(ɛ-caprolactone) (PCL) controls as well, but to a lesser extent than to the PTMC films. In this case, no signs of erosion were observed. Human skin fibroblasts cultured on PTMC and PCL films as controls also adhered to the films but did not erode the surfaces. The effect of enzymes and reactive oxygen species that can be secreted by macrophages on the erosion process was assessed using aqueous solutions of cholesterol esterase, lipoprotein lipase, esterase, potassium superoxide, and hydrogen peroxide. The PTMC films eroded in aqueous enzyme solutions as well as in aqueous superoxide solutions. Cholesterol esterase and superoxide anion radicals seem to be most involved in the macrophage-mediated erosion of PTMC. This macrophage culture model is useful in assessing the influence of macrophages on the in vivo biodegradability of polymers and in elucidating the biodegradation mechanisms involved.

Keywords
Poly(trimethylene carbonate); Macrophages; Enzymatic degradation; Reactive oxygen species; Surface erosion
First Page Preview
Macrophage-mediated erosion of gamma irradiated poly(trimethylene carbonate) films
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 22, August 2009, Pages 3652–3661
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering