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The defined presentation of nanoparticles to cells and their surface controlled uptake

Paper ID Volume ID Publish Year Pages File Format Full-Text
9207 620 2009 5 PDF Available
Title
The defined presentation of nanoparticles to cells and their surface controlled uptake
Abstract

New approaches and standardized test procedures to study the impact of nanoparticles (NPs) on living cells are urgently needed for the evaluation of potential hazards relating human exposure to NPs. Here we study semiconductor quantum dots (QDs) preparation on solid surfaces and the subsequent internalization by cells seeded on top of the NP layer. Controlled densities of NPs are adsorbed to solid surfaces coated with extra-cellular macromolecules. The fraction of QDs taken up by the cells is assessed by automated fluorescence microscopy z-scans. We find that NPs aggregate during the uptake process, forming clusters inside cells that are able to enter the cell nucleus. NP uptake is dependent on surface functionalization and can be hindered by increasing the strength of the adhesion force between NPs and the surface. Studying time dependent uptake, we show that particles are able to exit cells.

Keywords
ECM; Epithelial cell; Image analysis; Nanoparticle; Nanotoxicity; Quantum dots
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 22, August 2009, Pages 3766–3770
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us