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Microfabricated silicon nitride membranes for hepatocyte sandwich culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
9221 621 2008 10 PDF Available
Title
Microfabricated silicon nitride membranes for hepatocyte sandwich culture
Abstract

We have developed a hepatocyte sandwich culture with improved mass transport properties based on ultra-thin microfabricated porous silicon nitride (Si3N4) membranes. The dimensions and uniformity of the membrane pores can be configurable, which confers more control over the mass transport. Instead of collagen gels used in conventional sandwich culture, we utilized galactose ligands immobilized on the Si3N4 membranes to support hepatocyte attachment and function in the sandwich culture. Diffusion studies using FITC-dextrans confirmed that mass transport of the microfabricated Si3N4 membrane based sandwich was significantly better than conventional collagen gel sandwich and can be configured by varying the porosity of the Si3N4 membrane. Hepatocytes cultured in the microfabricated Si3N4 membrane based sandwich culture exhibited earlier apical repolarization and biliary excretion, improved differentiated functions and enhanced drug sensitivity compared to hepatocytes cultured in a collagen gel sandwich. The Si3N4 membrane based sandwich culture allows for a systematic optimization of the mass transport properties of hepatocyte culture by changing the pore size and inter-pore distance. This will enable more effective drug testing applications where optimal mass transport is required for hepatocyte function maintenance and drug accessibility.

Keywords
Galactose immobilization; Porous Si3N4 membrane; Mass transport; Drug testing; Sandwich culture; Hepatocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 29, October 2008, Pages 3993–4002
Authors
, , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us