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Synthesis of streptavidin-FITC-conjugated core–shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
9222 621 2008 9 PDF Available
Title
Synthesis of streptavidin-FITC-conjugated core–shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes
Abstract

We explored the feasibility of magnetite (Fe3O4)-gold (Au) core–shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe3O4-Au core–shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4+ T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe3O4-Au nanocrystals successfully pulled down CD4+ T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases.

Keywords
Fe3O4-Au; Core–shell nanocrystals; Streptavidin; Magnetic activated cell separation; Fluorescence-activated cell sorting
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Synthesis of streptavidin-FITC-conjugated core–shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 29, October 2008, Pages 4003–4011
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us