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The effect of cholesterol on protein-coated gold nanoparticle binding to liquid crystal-supported models of cell membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
9233 622 2010 8 PDF Available
Title
The effect of cholesterol on protein-coated gold nanoparticle binding to liquid crystal-supported models of cell membranes
Abstract

We report an easily visualized liquid crystal (LC)-based system to study the biophysical interactions between protein-coated gold nanoparticles (AuNPs) and LC-supported cell membrane model. The model consists of mixed phospholipid/cholesterol monolayer self-assembled at aqueous–LC interface. Protein-coated AuNPs were found to disrupt the mixed phospholipid/cholesterol monolayer. As a result, orientational transitions of LCs were triggered and optical responses of LCs from dark to bright were observed. The mixed monolayers with higher cholesterol contents were found to be more susceptible to the disruption by protein-coated AuNPs, and hydrophobic interaction played a major role in the monolayer disruption. We also found that the time for non-specific binding of fibrinogen-coated AuNPs to the mixed phospholipid/cholesterol monolayer was similar to that of specific binding of neutravidin-coated AuNPs to the mixed phospholipid/biotin-capped phospholipid monolayer. Results obtained from this study may offer new understanding in the potential nanotoxicity pathway, where the biophysical interaction between nanomaterials and cell membrane is an important step.

Keywords
Phospholipids; Cholesterol; Gold nanoparticles; Liquid crystals; Proteins
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The effect of cholesterol on protein-coated gold nanoparticle binding to liquid crystal-supported models of cell membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 11, April 2010, Pages 3008–3015
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us