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The formulation of aptamer-coated paclitaxel–polylactide nanoconjugates and their targeting to cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
9237 622 2010 11 PDF Available
Title
The formulation of aptamer-coated paclitaxel–polylactide nanoconjugates and their targeting to cancer cells
Abstract

Paclitaxel–polylactide (Ptxl–PLA) conjugate nanoparticles, termed as nanoconjugates (NCs), were prepared through Ptxl/(BDI)ZnN(TMS)2 (BDI = 2-((2,6-diisopropylphenyl)-amido)-4-((2,6-diisopropylphenyl)-imino)-2-pentene)-mediated controlled polymerization of lactide (LA) followed by nanoprecipitation. Nanoprecipitation of Ptxl–PLA resulted in sub-100 nm NCs with monomodal particle distributions and low polydispersities. The sizes of Ptxl–PLA NCs could be precisely controlled by using appropriate water-miscible solvents and by controlling the concentration of Ptxl–PLA during nanoprecipitation. Co-precipitation of a mixture of PLA–PEG–PLA (PLA = 14 kDa; PEG = 5 kDa) and Ptxl–PLA in PBS resulted in NCs that could stay non-aggregated in PBS for an extended period of time. To develop solid formulations of NCs, we evaluated a series of lyoprotectants, aiming to identify candidates that could effectively reduce or eliminate NC aggregation during lyophilization. Albumin was found to be an excellent lyoprotectant for the preparation of NCs in solid form, allowing lyophilized NCs to be readily dispersed in PBS without noticeable aggregates. Aptamer–NCs bioconjugates were prepared and found to be able to effectively target prostate-specific membrane antigen in a cell-specific manner.

Keywords
Nanoparticles; Drug delivery; Nanomedicine; Paclitaxel; Cancer targeting; PSMA aptamer
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The formulation of aptamer-coated paclitaxel–polylactide nanoconjugates and their targeting to cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 11, April 2010, Pages 3043–3053
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us