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A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
9239 622 2010 8 PDF Available
Title
A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles
Abstract

The effects of titanium dioxide (TiO2) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO2 NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO2 NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein–protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO2 NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct–EBI, GRID database, a protein–protein interaction network was constructed, which provides highly integrated information exhibiting the protein–protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphorylation, which is negatively regulated by the protein kinase C epsilon (PKCɛ), is demonstrated. It is inferred that PKCɛ plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO2 NPs exposure.

Keywords
Titanium dioxide nanoparticles; Neural stem cells; Differentiation; Protein–protein interaction; Proteomics
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A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 11, April 2010, Pages 3063–3070
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us