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In vitro behavior of neural stem cells in response to different chemical functional groups

Paper ID Volume ID Publish Year Pages File Format Full-Text
9252 623 2009 9 PDF Available
Title
In vitro behavior of neural stem cells in response to different chemical functional groups
Abstract

Neural stem cells (NSCs) cultured on glass surfaces modified by different chemical groups, including hydroxyl (–OH), sulfonic (–SO3H), amino (–NH2), carboxyl (–COOH), mercapto (–SH) and methyl (–CH3) groups, are shown here to commit to phonotypes with extreme sensitivity to surface chemical groups. The adhering NSCs at the level of single cells exhibited morphological changes in response to different chemical groups. NSCs on –SO3H surfaces had the largest contact area and the most flattened morphology, while those on –CH3 surfaces exhibited the smallest contact area and the most rounded morphology. After 5 days of culture, the migration of NSCs from their original aggregates onto these test surfaces followed the trend: –NH2>–COOH = –SH≫–SO3H>–CH3>–OH. The expression of specific markers, including nestin, β-Tubulin-III, glial fibrillary acidic protein and O4, were used to examine NSCs lineage specification. The –SO3H surfaces favored NSCs differentiation into oligodendrocytes, while NSCs in contact with –COOH, –NH2, –SH and –CH3 had the ability to differentiate into neurons, astrocytes and oligodendrocytes. Compared to –COOH surfaces, –NH2 seemed to promote neuronal differentiation. These chemically modified surfaces exhibited regulation of NSCs on adhesion, migration and differentiation potential, providing chemical means for the design of biomaterials to direct NSCs lineage specification for neural tissue engineering.

Keywords
Neural stem cells; Surface chemistry; Adhesion; Migration; Differentiation
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In vitro behavior of neural stem cells in response to different chemical functional groups
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1036–1044
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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