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The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients

Paper ID Volume ID Publish Year Pages File Format Full-Text
9255 623 2009 5 PDF Available
Title
The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients
Abstract

Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and R1 mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to <120 μm2.

Keywords
Cell culture; Cell spreading; Cell viability; Plasma polymerisation
First Page Preview
The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1066–1070
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering