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Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system

Paper ID Volume ID Publish Year Pages File Format Full-Text
9269 623 2009 8 PDF Available
Title
Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
Abstract

Cellular internalization of functional nanoparticles that have optical and magnetic properties is very important in the cellular imaging and manipulation of specifically targeted biomolecules. In this study, a robust method to deliver functional nanoparticles and targeting antibodies into cells was suggested. The engineered protein G system, which contains an affinity tag and a cell penetration peptide in the N- and C-terminals, respectively, can capture surface-modified nanoparticles and antibodies without chemical reaction, and then non-invasively deliver them into the cells. Finally, gold-coated iron oxide nanoparticle/engineered protein G hybrid systems were successfully employed as multifunctional cargo systems for the targeting, imaging, and manipulation of mitochondria.

Keywords
Nanoparticle; Gold; Engineered protein; Intracellular delivery; Surface modification; Cell imaging
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Simultaneous intracellular delivery of targeting antibodies and functional nanoparticles with engineered protein G system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1197–1204
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us