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The effect of the nonionic block copolymer pluronic P85 on gene expression in mouse muscle and antigen-presenting cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
9273 623 2009 14 PDF Available
Title
The effect of the nonionic block copolymer pluronic P85 on gene expression in mouse muscle and antigen-presenting cells
Abstract

DNA vaccines can be greatly improved by polymer agents that simultaneously increase transgene expression and activate immunity. We describe here Pluronic P85 (P85), a triblock copolymer of ethylene oxide (EO) and propylene oxide (PO) EO26–PO40–EO26. Using a mouse model we demonstrate that co-administration of a bacterial plasmid DNA with P85 in a skeletal muscle greatly increases gene expression in the injection site and distant organs, especially the draining lymph nodes and spleen. The reporter expression colocalizes with the specific markers of myocytes and keratinocytes in the muscle, as well as dendritic cells (DCs) and macrophages in the muscle, lymph nodes and spleen. Furthermore, DNA/P85 and P85 alone increase the systemic expansion of CD11c+ (DC), and local expansion of CD11c+, CD14+ (macrophages) and CD49b+ (natural killer) cell populations. DNA/P85 (but not P85) also increases maturation of local DC (CD11c + CD86+, CD11c + CD80+, and CD11c + CD40+). We suggest that DNA/P85 promotes the activation and recruitment of the antigen-presenting cells, which further incorporate, express and carry the transgene to the immune system organs.

Keywords
Copolymer; Gene expression; Leukocyte; Pluronics
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The effect of the nonionic block copolymer pluronic P85 on gene expression in mouse muscle and antigen-presenting cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 6, February 2009, Pages 1232–1245
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us