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Dual therapeutic action of antibiotic-loaded nanosheets for the treatment of gastrointestinal tissue defects

Paper ID Volume ID Publish Year Pages File Format Full-Text
9290 624 2010 10 PDF Available
Title
Dual therapeutic action of antibiotic-loaded nanosheets for the treatment of gastrointestinal tissue defects
Abstract

An ultra-thin polymer film (nanosheet) composed of polysaccharides (i.e., polysaccharide nanosheet) provides sufficient adhesiveness, flexibility and robustness to act as an effective wound dressing. We have recently demonstrated the sealing effect of a nanosheet on a murine cecal puncture. Nevertheless, a small percentage of bacteria penetrated the nanosheet because of its ultra-thin structure. Here, we have developed an antibiotic-loaded nanosheet to inhibit bacterial penetration and investigated its therapeutic efficacy using a model of a murine cecal puncture. Tetracycline (TC) was sandwiched between a poly(vinylacetate) (PVAc) layer and the polysaccharide nanosheet (named PVAc-TC-nanosheet). Under physiological conditions, TC was released from the nanosheet for 6 h. Microscopic observation between the interface of the PVAc-TC-nanosheet and bacteria demonstrated how its potent anti-microbial effect was achieved. In vivo studies show that overlapping therapy with the PVAc-TC-nanosheet (thickness: 177 nm) significantly increases mouse survival rate after cecal puncture as well as suppressing an increase in the intraperitoneal bacterial count and leukocyte count.

Keywords
anti-microbial; In vivo test; Inflammation; Nanocomposite; Polysaccharide; Wound dressing
First Page Preview
Dual therapeutic action of antibiotic-loaded nanosheets for the treatment of gastrointestinal tissue defects
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 24, August 2010, Pages 6269–6278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering