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Design of graded biomimetic osteochondral composite scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
9320 626 2008 8 PDF Available
Title
Design of graded biomimetic osteochondral composite scaffolds
Abstract

With the ultimate goal to generate suitable materials for the repair of osteochondral defects, in this work we aimed at developing composite osteochondral scaffolds organized in different integrated layers, with features which are biomimetic for articular cartilage and subchondral bone and can differentially support formation of such tissues. A biologically inspired mineralization process was first developed to nucleate Mg-doped hydroxyapatite crystals on type I collagen fibers during their self-assembling. The resulting mineral phase was non-stoichiometric and amorphous, resembling chemico-physical features of newly deposited, natural bone matrix. A graded material was then generated, consisting of (i) a lower layer of the developed biomineralized collagen, corresponding to the subchondral bone, (ii) an upper layer of hyaluronic acid-charged collagen, mimicking the cartilaginous region, and (iii) an intermediate layer of the same nature as the biomineralized collagen, but with a lower extent of mineral, resembling the tidemark. The layers were stacked and freeze-dried to obtain an integrated monolithic composite. Culture of the material for 2 weeks after loading with articular chondrocytes yielded cartilaginous tissue formation selectively in the upper layer. Conversely, ectopic implantation in nude mice of the material after loading with bone marrow stromal cells resulted in bone formation which remained confined within the lower layer. In conclusion, we developed a composite material with cues which are biomimetic of an osteochondral tissue and with the capacity to differentially support cartilage and bone tissue generation. The results warrant testing of the material as a substitute for the repair of osteochondral lesions in orthotopic animal models.

Keywords
Biomimetic material; Biomineralization; Chondrocyte; Collagen; Composite; Magnesium
First Page Preview
Design of graded biomimetic osteochondral composite scaffolds
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 26, September 2008, Pages 3539–3546
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering