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Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
9325 626 2008 8 PDF Available
Title
Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells
Abstract

A promising new direction for contrast-enhanced magnetic resonance (MR) imaging involves tracking the migration and biodistribution of superparamagnetic iron oxide (SPIO)-labeled cells in vivo. Despite the large number of cell labeling studies that have been performed with SPIO particles of differing size and surface charge, it remains unclear which SPIO configuration provides optimal contrast in non-phagocytic cells. This is largely because contradictory findings have stemmed from the variability and imprecise control over surface charge, the general need and complexity of transfection and/or targeting agents, and the limited number of particle configurations examined in any given study. In the present study, we systematically evaluated the cellular uptake of SPIO in non-phagocytic T cells over a continuum of particle sizes ranging from 33 nm to nearly 1.5 μm, with precisely controlled surface properties, and without the need for transfection agents. SPIO labeling of T cells was analyzed by flow cytometry and contrast enhancement was determined by relaxometry. SPIO uptake was dose-dependent and exhibited sigmoidal charge dependence, which was shown to saturate at different levels of functionalization. Efficient labeling of cells was observed for particles up to 300 nm, however, micron-sized particle uptake was limited. Our results show that an unconventional highly cationic particle configuration at 107 nm maximized MR contrast of T cells, outperforming the widely utilized USPIO (<50 nm).

Keywords
Molecular imaging; MRI; Ultrasmall superparamagnetic iron oxide; Standard superparamagnetic iron oxide; Micron-sized paramagnetic iron oxide; Nanoparticles
First Page Preview
Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 26, September 2008, Pages 3583–3590
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering