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Poly(l-lysine) nanostructured particles for gene delivery and hormone stimulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
9355 627 2010 8 PDF Available
Title
Poly(l-lysine) nanostructured particles for gene delivery and hormone stimulation
Abstract

In this work, we designed replica particles based on poly (l-lysine) (PLL) polymers crosslinked via a homobifunctional linker to support coadsorption of a plasmid DNA and a peptide hormone for concurrent transfection and induction of a cellular function. PLL replica particles (PLLRP) were prepared by infiltrating polymer into mesoporous silica (MS) particles, crosslinking the adsorbed chains by using a homobifunctional crosslinker and finally removing the template particles. Moreover, we verified their cytotoxicity. Furthermore, based on this PLLRP gene delivery system, we simultaneously evaluated the melanin stimulation and gene expression in these cells by fluorescence microscopy. To further understand the bi-functionality, we labeled the SPT7pTL and PGA-α-MSH with YOYO-1 and Rhodamine, respectively, to follow its intracellular pathway by confocal microscopy. Our data suggests that the PLLRP is a promising vector for gene therapy and hormone stimulation.

Keywords
Mesoporous silica (MS); Poly (l-lysine) replica particles (PLLRP); Gene delivery; Hormone stimulation
First Page Preview
Poly(l-lysine) nanostructured particles for gene delivery and hormone stimulation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 7, March 2010, Pages 1699–1706
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering