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Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
9370 627 2010 9 PDF Available
Title
Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles
Abstract

Although siRNAs have surpassed expectations in experiments to alter gene expression in vitro, the lack of an efficient in vivo delivery system still remains a challenge in siRNA therapeutics development and has been recognized as a major hurdle for clinical applications. In this paper we describe an inorganic nanoparticle-based delivery system that is readily adaptable for in vivo systems. Layered double hydroxide (LDH) nanoparticles, a family of inorganic crystals, tightly bind, protect, and release siRNA molecules and deliver them efficiently to mammalian cells in vitro. The uptake of siRNA-loaded LDH nanoparticles occurs via endocytosis, whereby the nanoparticles dissolve due to the low pH in the endosome, thereby aiding endosomal escape into the cytoplasm. The influence of LDH nanoparticles on cell viability and proliferation is negligible at concentrations ≤0.050 mg mL−1, and a pronounced down-regulation of protein expression upon LDH mediated siRNA transfection of HEK293T cells is observed.

Keywords
siRNA delivery; Layered double hydroxide nanoparticles
First Page Preview
Efficient siRNA delivery to mammalian cells using layered double hydroxide nanoparticles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 7, March 2010, Pages 1821–1829
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering