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The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
9373 627 2010 8 PDF Available
Title
The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles
Abstract

The development of transfection enhancement of liposomes with attributes of high stability and easy handling in gene therapy is challenging. In this study, we report didodecyldimethylammonium bromide (DDAB, a cationic lipid) coated gold nanoparticles (DDAB-AuNPs), which can enhance the transfection efficiency generated by two kinds of commercially available cationic liposomes: Lipotap and DOTAP. It showed that DDAB-AuNPs at the optimal concentrations could produce more than 2 times increase when measuring the number of cells expressed green fluorescent protein and 48-fold increase for luciferase levels after transfection, respectively. The electrophoretic mobility shift assay (EMSA) and confocal laser scanning microscopy (CLSM) experiments showed that more DNA molecules binding to the lipoplexes after adding DDAB-AuNPs. In addition, the flow cytometry (FCM) results indicated that DDAB-AuNPs increased cellular uptake efficiency of DNA molecules, which might account for the enhancement of transfection efficiency. It has also been found that the DDAB-AuNPs could decrease the cytotoxicity of liposomes to the cells.

Keywords
Trasfection enhancer; Liposome; Gold nanoparticles
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The enhancement of transfection efficiency of cationic liposomes by didodecyldimethylammonium bromide coated gold nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 7, March 2010, Pages 1850–1857
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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