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Fluorescent oligonucleotide probe based on G-quadruplex scaffold for signal-on ultrasensitive protein assay

Paper ID Volume ID Publish Year Pages File Format Full-Text
9381 627 2010 7 PDF Available
Title
Fluorescent oligonucleotide probe based on G-quadruplex scaffold for signal-on ultrasensitive protein assay
Abstract

This work reported the G-quadruplex structure of pyrene-labeled G-rich DNA probe and its application in the immunoglobulin E (IgE) detection, providing plausibly an insight into the biological function of human telomere. Based on the intermolecular G-quadruplex, a terminal-single-pyrene-labeled oligonucleotide signaling probe was developed and a novel protein assay strategy was proposed via combining specific DNA cleavage by S1 nuclease with target-recognizing aptamer. This assay platform not only circumvented the optimization of specific sites for reporter attachment and the pyrene monomer fluorescence quenching by flanking nucleotide bases but also presented a signal-on mechanism. Thus, ultrasensitive homogeneous detection of IgE was successfully conducted. A linear dynamic range of 4.72 × 10−12 to 7.56 × 10−9m, a regression coefficient of 0.9941 and a detection limit of 9.45 × 10−14m were given. Additionally, a preliminary concept of the single-pyrene-labeled excimer fluorescence probes associated with G-quadruplex for screening biological markers was described. Importantly, the unexpected structural property of G-quadruplex discovered seems to provide valuable information to allow understanding of the structure and function of human telomere and exploring of useful structure-based anticancer drug.

Keywords
G-quadruplex; Aptamer; Single-pyrene-labeled signaling probe; S1 nuclease; IgE
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Fluorescent oligonucleotide probe based on G-quadruplex scaffold for signal-on ultrasensitive protein assay
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 7, March 2010, Pages 1918–1924
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us