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Live cell-based sensor cells ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
9387 628 2010 5 PDF Available
Title
Live cell-based sensor cells ☆
Abstract

Biosensors utilizing live cells have attracted a great deal of attention because of their high specificity and sensitivity to their targets. We established cytotoxic sensor cells that were transfected with GFP plasmids derived from HSP70B′ and BTG2 promoters. These sensor cells have significant advantages in both speed and sensitivity for the detection of cytotoxicity. We created a live cell-based sensorchip fabricated with microfluidic channels. Fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels. With this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive, and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of μ-TAS technology that can be applied to numerous fields such as biomaterials, nanotoxicology, environmental assessment, and drug screening.

Keywords
Cell signaling; Cell viability; Gene expression; Genetic engineering
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Live cell-based sensor cells ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 23, August 2010, Pages 5911–5915
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us