fulltext.study @t Gmail

Modified polyelectrolyte complex fibrous scaffold as a matrix for 3D cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
9389 628 2010 9 PDF Available
Title
Modified polyelectrolyte complex fibrous scaffold as a matrix for 3D cell culture
Abstract

The paradigm of scaffold tissue engineering relies on the provision of an appropriate environment for cell growth, which includes both structural support and the presentation of cellular signals. In terms of biosignal presentation, fibrous scaffolds by interfacial polyelectrolyte complexation (IPC) offer a clear advantage over other scaffold types as IPC scaffolds are formed using an aqueous-based, room-temperature process compatible with the incorporation of biological molecules. This paper establishes two primary methods for the chemical and biochemical modification of these scaffolds: (i) physical entrapment of the bioactive component, and (ii) covalent binding of the bioactive component. For the first method, extracellular matrix (ECM) proteins, collagen, fibronectin and laminin were drawn into the IPC fiber. For the second method, the cell adhesion peptide, RGD, was chemically conjugated to a thiol-active maleimidylated form of the scaffold. Immobilization of the bioactive components was characterized by confocal fluorescence microscopy, scanning electron microscopy (SEM) and BCA protein assay. The ECM proteins were distributed throughout the bulk and surface of the fiber. The ratio of covalently bound to physisorbed RGD was ∼ 2:3. The performance of the various scaffolds as a matrix to maintain the differentiated function of primary hepatocytes showed that albumin levels in the supernatant were in the order of RGD-modified scaffold > collagen Type I-modified scaffold > fibronectin- or laminin-modified scaffold > unmodified scaffold > plate, while no clear trend in urea production could be discerned. Thus, IPC scaffolds offered a promising platform for the presentation of signals to cells, in this case, to influence their differentiated function.

Keywords
ECM (extracellular matrix); RGD peptide; Scaffold; Non-woven fabric; Hepatocyte; Cell culture
First Page Preview
Modified polyelectrolyte complex fibrous scaffold as a matrix for 3D cell culture
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 23, August 2010, Pages 5927–5935
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us