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An optical biosensing platform for proteinase activity using gold nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
9406 628 2010 9 PDF Available
Title
An optical biosensing platform for proteinase activity using gold nanoparticles
Abstract

The surface plasmon resonance (SPR) wavelength of colloidal gold nanoparticles (AuNPs) can vary when the AuNPs aggregate, have different sizes or shapes, or are modified with chemical molecules. In this study, an optical biosensing platform for a proteinase activity assay was established based on the SPR property of AuNPs. The 13-nm AuNPs were modified with gelatin (AuNPs-gelatin) as a proteinase substrate and subsequently modified with 6-mercaptohexan-1-ol (MCH) (AuNPs/MCH-gelatin). After proteinase (trypsin or gelatinase) digestion, the AuNPs lose shelter, and MCH increases the attractive force between the modified AuNPs. Therefore, the AuNPs gradually move closer to each other, resulting in AuNPs aggregation. The AuNPs aggregation can be monitored by the red shift of surface plasmon absorption and a visible color change of the AuNPs is from red to blue. Such a color change can be observed with the naked eye. For detection, the absorption ratio, A625/A525, of the reacted AuNPs solution can be used to estimate quantitatively the proteinase activity. A linear correlation has been established with trypsin activity at concentrations from 1.25 × 10−1 to 1.25 × 102 U and matrix metalloproteinase-2 activity at concentrations from 50 ng/mL to 600 ng/mL.

Keywords
6-Mercaptohexan-1-ol; Gold nanoparticles; Matrix metalloproteinase; Optical biosensor; Proteinase
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An optical biosensing platform for proteinase activity using gold nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 23, August 2010, Pages 6087–6095
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us