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Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival

Paper ID Volume ID Publish Year Pages File Format Full-Text
9407 628 2010 8 PDF Available
Title
Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival
Abstract

A biopolymer has been shown to facilitate efficient delivery of trehalose, a bioprotectant normally impermeable to the phospholipid bilayer, into ovine erythrocytes. Cellular uptake of trehalose was found to be dependent on polymer pendant amino acid type and degree of grafting, polymer concentration, pH, external trehalose concentration, incubation temperature and time. Optimization of these parameters yielded an intracellular trehalose concentration of 123 ± 16 mm and concomitant improvement of erythrocyte cryosurvival of up to 20.4 ± 5.6%. Intracellular trehalose was shown to impart cellular osmoprotection up to an external osmolarity of 230 mOsm and increased osmotic sensitivity above this threshold. Biopolymer mediated membrane permeability was shown to be rapidly and completely reversible via washing with phosphate buffered saline.

Keywords
Biopolymer; Blood; Cryopreservation; Trehalose; Cell viability
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Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 23, August 2010, Pages 6096–6103
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us