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The influence of pancreas-derived stem cells on scaffold based skin regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
9422 629 2009 8 PDF Available
Title
The influence of pancreas-derived stem cells on scaffold based skin regeneration
Abstract

It has been shown that Pancreatic Stem Cells (PSCs) share many features with skin stem cells. Yet, their potential role in skin regeneration remains to be elucidated. 5 × 105 PSCs from male rattus norwegicus were seeded on Matriderm® scaffold overnight. Cells survival and proliferation were then tested in vitro showing the survival of the cells and their homogenous distribution in the scaffolds. Afterwards, scaffolds were used to replace bilateral full-thickness skin wounds made on the dorsum of Nu/Nu mice. A control group of nude mice received the Matriderm scaffolds without cells. Two weeks after transplantation, wound areas were harvested and analyzed with respect to epithelialization, vascularization and wound closure. The healing area and regeneration rate were significantly increased in the group used the PSCs-seeded scaffolds (factor of 2.1). Vascularization rate showed a significant increase in the PSCs-seeded scaffolds(factor of 1.5). Morphology and immunohistochemistry showed new skin-like structures positive to epidermal markers in the healing wound bed. PSCs were detected in the regenerated tissues. This study showed that the combined use of PSCs with the Matriderm as a scaffold for dermal regeneration significantly increased the epidermalization, vascularization and healing in full-thickness wounds.

Keywords
Tissue engineering; Pancreas; Stem cells; Wound Healing; Scaffolds
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The influence of pancreas-derived stem cells on scaffold based skin regeneration
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 5, February 2009, Pages 789–796
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us