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The modification of quantum dot probes used for the targeted imaging of his-tagged fusion proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
9428 629 2009 7 PDF Available
Title
The modification of quantum dot probes used for the targeted imaging of his-tagged fusion proteins
Abstract

In molecular biology and protein detection the immobilized metal ion clusters using a NTA-chelator is a powerful technique in identification and isolation of histidine-tagged fusion proteins. The Oligo-histidine tag should serve as a high affinity binding sequence for the purification of any fusion protein via metal chelating adsorbents. We described the preparation and characterization of bioinorganic conjugates made with highly luminescent semiconductor CdTe–CdS core–shell quantum dots (QDs) for biological labeling. A biocompatible surface-functionalized nanoparticle was designed to sense histidine-tagged fusion proteins. This study demonstrates the synthesis of Ni–NTA conjugated QD nanoparticles and the successful application of these nanoparticles to the detection of histidine-tagged fusion proteins. It is believed that this approach will provide a more convenient methodology for the intracellular localization of histidine-tagged protein, as compared with current methods. These Ni–NTA–QD clusters were shown to target the 6x histidine region of tagged proteins specially.

Keywords
CdTe; Quantum dot; Nickel; Bio-imaging
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The modification of quantum dot probes used for the targeted imaging of his-tagged fusion proteins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 5, February 2009, Pages 836–842
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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