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Versatile supramolecular pDNA vehicles via “click polymerization” of β-cyclodextrin with oligoethyleneamines

Paper ID Volume ID Publish Year Pages File Format Full-Text
9438 629 2009 11 PDF Available
Title
Versatile supramolecular pDNA vehicles via “click polymerization” of β-cyclodextrin with oligoethyleneamines
Abstract

Herein, we report the efficient synthesis of high molecular weight polymers (up to 331 kDa) that contain β-cyclodextrin within the polymer backbone and the examination of these structures for pDNA delivery within cultured mammalian cells. Two series of polymers were synthesized, one with variation in oligoethyleneamine stoichiometry, Cd146, Cd244, Cd349, and Cd447 (1-4 oligoethyleneamines in the repeat unit, respectively and similar degree of polymerization, nw = 44–49) and another with variation in polymer length (four ethyleneamines in the repeat unit), Cd427, Cd447, Cd493, and Cd4200 [nw = 27, 47, 93, 200] via the “click reaction”. The two series of polymers revealed efficient pDNA binding and compaction through gel electrophoresis, dynamic light scattering, and transmission electron microscopy experiments. The DNase protection assay showed a decrease in pDNA degradation with an increase in the polymer amine stoichiometry, where polymer Cd349 and all of the Cd4 analogs completely protected pDNA for up to 8 h in serum. The cellular uptake and gene expression profiles were examined in HeLa cells, which similarly demonstrated that both the series of polymers had high pDNA delivery where, Cd349 and Cd493 had the most effective luciferase gene expression. In addition, the cell viability profiles were quite high with all of the structures.

Keywords
Biocompatibility; Cytotoxicity; DNA; Copper; Nanoparticle; Polymerization
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Versatile supramolecular pDNA vehicles via “click polymerization” of β-cyclodextrin with oligoethyleneamines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 5, February 2009, Pages 928–938
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us