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Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release

Paper ID Volume ID Publish Year Pages File Format Full-Text
9445 630 2008 6 PDF Available
Title
Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release
Abstract

The ability of nitric oxide (NO)-releasing xerogels to reduce fibrinogen-mediated adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli is described. A negative correlation was observed between NO surface flux and bacterial adhesion for each species tested. For S. aureus and E. coli, reduced adhesion correlated directly with NO flux from 0 to 30 pmol cm−2 s−1. A similar dependence for S. epidermidis was evident from 18 to 30 pmol cm−2 s−1. At a NO flux of 30 pmol cm−2 s−1, surface coverage of S. aureus, S. epidermidis, and E. coli was reduced by 96, 48, and 88%, respectively, compared to non-NO-releasing controls. Polymeric NO release was thus demonstrated to be an effective approach for significantly reducing fibrinogen-mediated adhesion of both gram-positive and gram-negative bacteria in vitro, thereby illustrating the advantage of active NO release as a strategy for inhibiting bacterial adhesion in the presence of pre-adsorbed protein.

Keywords
Polymeric nitric oxide release; Bacterial adhesion; Fibrinogen; Antimicrobial
First Page Preview
Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 30, October 2008, Pages 4039–4044
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering