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Redistribution of P-selectin ligands on neutrophil cell membranes and the formation of platelet–neutrophil complex induced by hemodialysis membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
9464 631 2008 7 PDF Available
Title
Redistribution of P-selectin ligands on neutrophil cell membranes and the formation of platelet–neutrophil complex induced by hemodialysis membranes
Abstract

The formation of platelet–neutrophil microaggregates and successive activation of neutrophils are closely related to hemodialysis-associated complications. The microaggregate is mediated primarily by the interaction between P-selectin (CD62P) expressed on activated platelets and P-selectin glycoprotein ligand-1 (PSGL-1, CD162) expressed on neutrophils. We previously reported that the clustered distribution of PSGL-1 on the cell membranes of chemokine-treated neutrophils caused upregulation of the microaggregate formation. In this study, we found that neutrophils treated with human plasma that had been incubated with hemodialysis membranes greatly enhanced the microaggregate formation. The membrane-treated plasma also induced PSGL-1 to form a cap-like cluster on the neutrophil surface. Analysis of several hemodialysis membranes with different materials indicated that the inducibility for the cap-like cluster formation of PSGL-1 parallels their ability to activate the complement system. Both the enhancement of microaggregate formation and the redistribution of PSGL-1 induced by the hemodialysis membrane-treated plasma were almost completely abrogated in the presence of a specific antagonist for the complement component C5a receptor, W-54011. These results strongly suggest that the generation of anaphylatoxin C5a through complement activation induced by hemodialysis membranes is responsible for the clustered redistribution of PSGL-1 in neutrophils leading to the increase in the platelet–neutrophil microaggregate formation. The present study indicates the importance of synergistic exacerbation of complement activation and platelet–neutrophil microaggregate formation in developing hemodialysis-associated complications.

Keywords
Anaphylatoxin; Cell adhesion; Complement; Hemodialysis; Platelet–neutrophil microaggregate; P-selectin glycoprotein ligand-1C3a, complement component C3a; C5a, complement component C5a; CDA, cellulose diacetate; CTA, cellulose triacetate; EVAL, ethylene v
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Redistribution of P-selectin ligands on neutrophil cell membranes and the formation of platelet–neutrophil complex induced by hemodialysis membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 21, July 2008, Pages 3084–3090
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us