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The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite–PCL composites

Paper ID Volume ID Publish Year Pages File Format Full-Text
9495 633 2010 12 PDF Available
Title
The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite–PCL composites
Abstract

We developed a composite biphasic calcium phosphate (BCP) scaffold by coating a nanocomposite layer, consisting of hydroxyapatite (HA) nanoparticles and polycaprolactone (PCL), over the surface of BCP. The effects of HA particle size and shape in the coating layer on the mechanical and biological properties of the BCP scaffold were examined. Micro-computerized tomography studies showed that the prepared scaffolds were highly porous (∼91%) with large pore size (400–700 μm) and an interconnected porous network of ∼100%. The HA nanoparticle (needle shape)-composite coated scaffolds displayed the highest compressive strength (2.1 ± 0.17 MPa), compared to pure HA/β-TCP (0.1 ± 0.05 MPa) and to the micron HA – composite coated scaffolds (0.29 ± 0.07 MPa). These needle shaped scaffolds also showed enhanced elasticity and similar stress–strain profile to natural bone. Needle shaped coated HA/PCL particles induced the differentiation of primary human bone derived cells, with significant upregulation of osteogenic gene expression (Runx2, collagen type I, osteocalcin and bone sialoprotein) and alkaline phosphatase activity compared to other groups. These properties are essential for enhancing bone ingrowth in load-bearing applications. The developed composite scaffolds possessed superior physical, mechanical, elastic and biological properties rendering them potentially useful for bone tissue regeneration.

Keywords
Bone; Scaffolds; Hydroxyapatite; Polymer
First Page Preview
The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite–PCL composites
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 21, July 2010, Pages 5498–5509
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering