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In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands

Paper ID Volume ID Publish Year Pages File Format Full-Text
9497 633 2010 6 PDF Available
Title
In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands
Abstract

Despite the rapid development of engineered skin, such skin still lacks skin appendages. Sweat glands, one of the skin appendages, play key roles in the maintenance of homeostasis and temperature regulation. In this study, we tested whether sweat glands could be integrated into engineered skin constructs to improve the quality of tissue regeneration. Using gelatin microspheres(containing epidermal growth factor [EGF]) as multifunctional vehicles, we cultured sweat gland cells (SGCs) on them and delivered SGCs-microspheres complex (SMC) into the engineered skin construct, which was created in vitro by culturing human keratinocytes on top of a fibroblast-embedded collagen-based matrix in an organotypic co-culture model. This engineered skin construct was then transplanted onto full-thickness cutaneous wounds in an athymic murine model. EGF-loaded microspheres displayed more cellular growth-promoting efficiency, and thus SMC was an available means for SGCs delivery. Constitution of the engineered skin constructs formed a skin-like pattern in vitro. Remarkably, SMC could differentiate toward a sweat gland-like structure in vitro within the hybrid matrix. Furthermore, the degree of wound healing in mice with this skin construct implantation was better than that with controls. This engineered skin construct could be used as a promising tool for regeneration of sweat glands in skin repair and a valuable engineered strategy for constitution of appendage-containing engineered skin models.

Keywords
Engineered skin; Sweat glands; Collagen; Epidermal growth factor; Wound healing
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In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 21, July 2010, Pages 5520–5525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us