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Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials

Paper ID Volume ID Publish Year Pages File Format Full-Text
9523 634 2009 7 PDF Available
Title
Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials
Abstract

Biphasic calcium phosphate (BCP), a mixture of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), has attracted attention as an excellent bone graft substitute. Mixtures of ceramics with agarose, as natural biodegradable binder, have been recently performed in order to increase the flexibility of the ceramic component and to facilitate the biomaterial preparation. In previous studies we have evaluated the response of both L929 fibroblasts and Saos-2 osteoblasts to hydroxyapatite–βTCP/agarose disks observing a higher sensitivity of osteoblasts to this biomaterial. In the present study, the use of specific fluorescent probes and antibodies has allowed to evaluate different cell function parameters as biocompatibility markers for the cell/biomaterial interaction of Saos-2 osteoblasts cultured for 7 days on hydroxyapatite–βTCP/agarose disks. The cell cycle subG1 fraction, the exposition of phosphatidylserine on the outside surface of the plasma membrane and the analysis of plasma membrane integrity versus cell size, indicate that the interaction with the biomaterial induces a light increase of apoptosis in osteoblasts without producing cell necrosis. The high percentage of viable cells on the biomaterial and the preservation of endothelial nitric oxide synthase (eNOS) expression, eNOS activity and mitochondrial membrane potential (Δψm), demonstrate the good biocompatibility of hydroxyapatite–βTCP/agarose disks and its potential utility for bone substitution and repair.

Keywords
Biphasic calcium phosphate; Agarose; Bone graft; Saos-2 osteoblasts; Nitric oxide
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Biocompatibility markers for the study of interactions between osteoblasts and composite biomaterials
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 1, January 2009, Pages 45–51
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us