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The effect of extensible PEG tethers on shielding between grafted thermo-responsive polymer chains and integrin–RGD binding

Paper ID Volume ID Publish Year Pages File Format Full-Text
9538 635 2008 6 PDF Available
Title
The effect of extensible PEG tethers on shielding between grafted thermo-responsive polymer chains and integrin–RGD binding
Abstract

The affinity control of integrin–RGD (Arg-Gly-Asp) binding by a thermal “on–off” switch has been achieved using newly designed surfaces presenting grafted temperature-responsive poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) copolymers functionalized with synthetic peptides. The prepared surface was designed to expose the tethered peptides available for cell binding at active “on” state above the lower critical solution temperature (LCST). The fully extended chains, on the other hand, masked the peptides completely and the cells started to detach from the surfaces at inactive “off” sate below the LCST. This paper elucidates the shielding effect of the grafted polymer chains on the dissociation of integrin–RGD binding below the LCST. To assess the ability of the polymer-shielding, extensible poly(ethylene glycol) (PEG) tethers were introduced between peptides and the grafted polymers. PEG chains allow peptides to be tethered to surfaces via functional PEG end-groups, leading to active “on” state even below the LCST. The time required to release cells from the surface was found to be longer when peptides were coupled to an extensible tether ends, suggesting that the surfaces can engender cell attachment through adhesive moieties covalently bound to the free ends of PEG chains. These results indicate that architectural changes on the nanometer length scale are crucial for controlling integrin–RGD binding and one of the main factors causing cell detachment is the shielding effect of the grafted polymer chains.

Keywords
Poly(N-isopropylacrylamide); 2-Carboxyisopropylacrylamide; Temperature-responsive surface; RGD peptides; Affinity control
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The effect of extensible PEG tethers on shielding between grafted thermo-responsive polymer chains and integrin–RGD binding
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 27, September 2008, Pages 3650–3655
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us