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The effect of lactose-conjugated silk biomaterials on the development of fibrogenic fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
9562 636 2008 11 PDF Available
Title
The effect of lactose-conjugated silk biomaterials on the development of fibrogenic fibroblasts
Abstract

Surface properties of implanted biomaterials can cause fibrotic tissue reactions by stimulating differentiation of host fibroblasts into contractile myofibroblasts. Silk fibroin (SF) protein has been used as biomaterial in pure and blended form. however, its effect on myofibroblast differentiation remains elusive. We here conjugated SF with lactose using cyanuric chloride as coupling spacer. NMR spectroscopy and the conjugates ability to agglomerate Abrus precatorius agglutinin verified efficient conjugation. Two-dimensional films and three-dimensional scaffolds produced from pure and lactose-conjugated SF solutions were tested as culture substrates for subcutaneous fibroblasts and myofibroblasts. Lactose-conjugated SF substrates mediated higher adhesion, proliferation and viability of fibroblastic cells than pure SF. This SF film composition promotes better attachment of fibroblasts than myofibroblasts. Pro-fibrotic cytokine TGFβ1 was ineffective in inducing fibroblast-to-myofibroblast differentiation on such substrates. Pre-differentiated myofibroblasts lost their contractile phenotype within a few days of being cultured on lactose-conjugated SF. Myofibroblast differentiation was also suppressed by growth in three-dimensional lactose-conjugated SF scaffolds that, however, support population with fibroblasts. We propose that this biomaterial will promote tissue integration without causing a fibrotic host reaction.

Keywords
Film; Scaffold; Lactose; Silk fibroin; Myofibroblastα-SMA, α-smooth muscle actin; ConA, concanavalin A; CY, cyanuric chloride; ECM, extracellular matrix; FA, focal adhesion; Lac–CY–SF, lactose–cyanuric chloride–silk fibroin conjugates; Lac, lactose; RGD,
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The effect of lactose-conjugated silk biomaterials on the development of fibrogenic fibroblasts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 35, December 2008, Pages 4665–4675
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us