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The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
9584 637 2008 12 PDF Available
Title
The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells
Abstract

In this study we present the enzymatic and biological analysis of polysialic acid (polySia) based hydrogel in terms of its degradation and cytocompatibility. PolySia based hydrogel is completely degradable by endosialidase enzyme which may avoid second surgery after tissue recovery. Viability assay showed that soluble components of polySia hydrogel did not cause any toxic effect on cultured Schwann cells. Moreover, green fluorescence protein transfected neonatal and adult Schwann cells, neural stem cells and dorsal root ganglionic cells (unlabelled) were seeded on polySia hydrogel modified with poly-l-lysine (Pll), poly-l-ornithine–laminin (porn–laminin) or collagen. Water soluble tetrazolium salt assay revealed that modification of the hydrogel significantly improved cell adhesion and viability. These results infer that polySia based scaffolds in combination with cell adhesion molecules and cells genetically modified to express growth factors would potentially be promising alternative in reconstructive therapeutic strategies.

Keywords
Polysialic acid hydrogel; Schwann cells; Dorsal root ganglia cells; Neural stem cells; Surface modification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 12, April 2008, Pages 1880–1891
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us