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The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation

Paper ID Volume ID Publish Year Pages File Format Full-Text
9585 637 2008 9 PDF Available
Title
The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation
Abstract

The capacity to deliver, temporally, bioactive growth factors in combination with appropriate progenitor and stem cells to sites of tissue regeneration promoting angiogenesis and osteogenesis offers therapeutic opportunities in regenerative medicine. We have examined the bone regenerative potential of encapsulated vascular endothelial growth factor (VEGF165) biodegradable poly(dl-lactic acid) (PLA) scaffolds created using supercritical CO2 fluid technology to encapsulate and release solvent-sensitive and thermolabile growth factors in combination with human bone marrow stromal cells (HBMSC) implanted in a mouse femur segmental defect (5 mm) for 4 weeks. HBMSC seeded on VEGF encapsulated PLA scaffolds showed significant bone regeneration in the femur segmental defect compared to the scaffold alone and scaffold seeded with HBMSC as analysed by indices of increased bone volume (BV mm3), trabecular number (Tb.N/mm) and reduced trabecular separation (Tb.Sp. mm) in the defect region using micro-computed tomography. Histological examination confirmed significant new bone matrix in the HBMSC seeded VEGF encapsulated scaffold group as evidenced by Sirius red/alcian blue and Goldner's trichrome staining and type I collagen immunocytochemistry expression in comparison to the other groups. These studies demonstrate the ability to deliver, temporally, a combination of VEGF released from scaffolds with seeded HBMSC to sites of bone defects, results in enhanced regeneration of a bone defect.

Keywords
Bone tissue regeneration; Supercritical fluids; PLA scaffolds; VEGF; Segmental femur defect; Human bone marrow stromal cells
First Page Preview
The effect of mesenchymal populations and vascular endothelial growth factor delivered from biodegradable polymer scaffolds on bone formation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 12, April 2008, Pages 1892–1900
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering