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The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype

Paper ID Volume ID Publish Year Pages File Format Full-Text
9610 638 2009 9 PDF Available
Title
The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype
Abstract

Poly(ethylene glycol) diacrylate (PEGDA) hydrogel scaffolds were engineered to promote contractile smooth muscle cell (SMC) phenotype via controlled release of heparin. The scaffold design was evaluated by quantifying the effects of free heparin on SMC phenotype, engineering hydrogels to provide controlled release of heparin, and synthesizing cell-adhesive, heparin releasing hydrogels to promote contractile SMC phenotype. Heparin inhibited SMC proliferation and up-regulated expression of contractile SMC phenotype markers, including smooth muscle α-actin, calponin, and SM-22α, in a dose-dependent fashion (6 μg/ml to 3.2 mg/ml). Heparin release from PEGDA hydrogels was controlled by altering PEGDA molecular weight (MW 1000–6000) and concentration at polymerization (10–30% w/w), yielding release profiles ranging from hours to weeks in duration. Heparin released from PEGDA gels, formulated for optimized heparin loading and release kinetics (30% w/w PEGDA, MW 3000), stimulated SMCs to up-regulate contractile marker mRNA. A cell-instructive scaffold construct was prepared by polymerizing a thin hydrogel film, with pendant RGD peptides for cell attachment, over the optimized hydrogel depots. SMCs seeded on these constructs had elevated levels of contractile marker mRNA after 3 d of culture compared with SMCs on control constructs. These results indicate that RGD-modified, heparin releasing PEGDA gels can act as cell-instructive scaffolds that promote contractile SMC phenotype.

Keywords
Smooth muscle cell; Arterial tissue engineering; Hydrogel; Scaffold; Vascular graft
First Page Preview
The effects of heparin releasing hydrogels on vascular smooth muscle cell phenotype
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 31, October 2009, Pages 6286–6294
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering