fulltext.study @t Gmail

The use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
9611 638 2009 14 PDF Available
Title
The use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation
Abstract

An injectable and thermosensitive poly(organophosphazene)–RGD conjugate to enhance functionality was synthesized by a covalent amide linkage between a cell adhesion peptide, GRGDS and carboxylic acid-terminated poly(organophosphazene). The aqueous solutions of synthesized poly(organophosphazene)–GRGDS conjugates existed in an injectable fluid state at room temperature and immediately formed a hydrogel at body temperature. The rabbit mesenchymal stem cells (rMSCs) on the polymer–GRGDS conjugate (conjugate 1-2, 0.05 mol fraction as GRGDS) hydrogel constructs using an injection method into a nude mouse were proved to express markers at mRNA level for all stages towards osteogenesis and mainly a sharp increase of osteocalcin (OCN, a typical late osteogenic differentiation marker) levels at 4th week post-induction indicated that the maturation process has started within this period. By histological and immunohistochemical evaluations, significantly high mineralization level by calcium contents was detected qualitatively and collagen type I (Col I), a major characteristic marker protein, was mainly and highly expressed by the rMSCs cultivated in the polymer–GRGDS conjugate hydrogel constructs formed into the nude mouse. The results suggest that the poly(organophosphazene)–GRGDS conjugate to enhance biofunctionality hold a promise for cell delivery material to induce osteogenic differentiation of MSC for enhancing ectopic bone formation.

Keywords
Thermosensitive; Injectable; Polyphosphazene–RGD conjugate; Mesenchymal stem cells (MSCs); Osteogenic differentiation; Hydrogel
First Page Preview
The use of injectable, thermosensitive poly(organophosphazene)–RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 31, October 2009, Pages 6295–6308
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us