fulltext.study @t Gmail

Neuronal polarity selection by topography-induced focal adhesion control

Paper ID Volume ID Publish Year Pages File Format Full-Text
9630 639 2010 13 PDF Available
Title
Neuronal polarity selection by topography-induced focal adhesion control
Abstract

Interaction between differentiating neurons and the extracellular environment guides the establishment of cell polarity during nervous system development. Developing neurons read the physical properties of the local substrate in a contact-dependent manner and retrieve essential guidance cues. In previous works we demonstrated that PC12 cell interaction with nanogratings (alternating lines of ridges and grooves of submicron size) promotes bipolarity and alignment to the substrate topography. Here, we investigate the role of focal adhesions, cell contractility, and actin dynamics in this process. Exploiting nanoimprint lithography techniques and a cyclic olefin copolymer, we engineered biocompatible nanostructured substrates designed for high-resolution live-cell microscopy. Our results reveal that neuronal polarization and contact guidance are based on a geometrical constraint of focal adhesions resulting in an angular modulation of their maturation and persistence. We report on ROCK1/2-myosin-II pathway activity and demonstrate that ROCK-mediated contractility contributes to polarity selection during neuronal differentiation. Importantly, the selection process confined the generation of actin-supported membrane protrusions and the initiation of new neurites at the poles. Maintenance of the established polarity was independent from NGF stimulation. Altogether our results imply that focal adhesions and cell contractility stably link the topographical configuration of the extracellular environment to a corresponding neuronal polarity state.

Keywords
Cell polarity; Neuron; Focal adhesions; Contact guidance; Topography; Cell contractility
First Page Preview
Neuronal polarity selection by topography-induced focal adhesion control
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 17, June 2010, Pages 4682–4694
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering