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The role of cyclic AMP in normalizing the function of engineered human blood microvessels in microfluidic collagen gels

Paper ID Volume ID Publish Year Pages File Format Full-Text
9632 639 2010 9 PDF Available
Title
The role of cyclic AMP in normalizing the function of engineered human blood microvessels in microfluidic collagen gels
Abstract

Nearly all engineered tissues must eventually be vascularized to survive. To this end, we and others have recently developed methods to synthesize extracellular matrix-based scaffolds that contain open microfluidic networks. These scaffolds serve as templates for the formation of endothelial tubes that can be perfused; whether such microvascular structures are stable and/or functional is largely unknown. Here, we show that compounds that elevate intracellular concentrations of the second messenger cyclic AMP (cAMP) strongly normalize the phenotype of engineered human microvessels in microfluidic type I collagen gels. Cyclic AMP-elevating agents promoted vascular stability and barrier function, and reduced cellular turnover. Under conditions that induced the highest levels of cAMP, the physiology of engineered microvessels in vitro quantitatively mirrored that of native vessels in vivo. Computational analysis indicated that cAMP stabilized vessels partly via its enhancement of barrier function.

Keywords
Microvascular tissue engineering; Collagen gels; Permeability; Microfluidic channels; Cyclic AMP
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The role of cyclic AMP in normalizing the function of engineered human blood microvessels in microfluidic collagen gels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 17, June 2010, Pages 4706–4714
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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