fulltext.study @t Gmail

The role of heparin self-association in the gelation of heparin-functionalized polymers

Paper ID Volume ID Publish Year Pages File Format Full-Text
9668 642 2008 8 PDF Available
Title
The role of heparin self-association in the gelation of heparin-functionalized polymers
Abstract

The extensive use of the glycosaminoglycan (GAG) heparin in the design of emerging biomaterials has made the physical characterization of this heterogeneous biomacromolecule increasingly important. In this work, heparin solutions are characterized via dynamic light scattering to investigate heparin's self-association, since this behavior was recently hypothesized to play a role in the gelation of heparin-functionalized polymer hydrogels. Samples of either low molecular weight heparin or high molecular weight heparin were filtered using membranes with 100, 220, or 450 nm average pore sizes. The 100 and 220 nm filters produce a single population of monomers with a diameter range of 3–10 nm in the intensity-weighted size distribution. However, the 450 nm filters reveal a second population of associated heparin. Increasing the solution concentration of high molecular weight heparin (HMWH) from 2.5 to 10 wt% causes the magnitude of the smaller population to decrease, while the diameter of the larger associated species approximately doubles. HMWH from different manufacturers displays varying degrees of association. Therefore, weaker associating HMWH can potentially be identified to control heparin self-interactions. Finally, fractionated, N-deacetylated low molecular weight heparin (LMWH) is compared to unmodified LMWH. The chemically modified heparin exhibits a heightened degree of association, suggesting an enhanced self-interaction. The increased negative charge of LMWH in the fractionated sample likely enhances polyelectrolyte interactions proposed to drive the association of these similarly charged polysaccharides. A more detailed understanding of heparin–heparin interactions will assist in the design of new scaffold materials with controlled release profiles, in the clinical use of heparin as an anticoagulant, and in investigations of interactions of other like-charged biomacromolecules.

Keywords
Hydrogel; Heparin; Mechanical properties; Rheology; Cross-linking
First Page Preview
The role of heparin self-association in the gelation of heparin-functionalized polymers
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 10, April 2008, Pages 1299–1306
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us